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Type test

OVERVIEW VAN DE TESTEN UITGEVOERD IN HET REFERENTIECENTRUM

Info

 

 
Purpose of the test
 Description of tests
As requested by routine laboratory
Surveillance
Technical validation
Confirmation of identification
Capsular (CPS) typing
Confirmation of Penicillin MIC
Epidemiological and microbial surveillances (CPS, pili, AST and virulence factors monitoring)
Specific study protocol
GBS diseases
Healthy carriers(1)
1- CULTURE
 
 
 
 
X
X
2- IDENTIFICATION TESTS used alone or in combination:
a- Biochemical tests
b-   Immunological detection of group B antigen
c- MALDI-TOF Mass Spectrometry
X
 
 
X(2)
X
X
3- DETERMINATION OF CAPSULAR (CPS) TYPE
a- Capsular serotyping
b-   Capsular genotyping (PCR)


(X)(2)
(X)(2)


X
X
 


X
(3)
X(3)


X
X


X
X
4- DETERMINATION OF ANTIMICROBIAL SUSCEPTIBILITY
a-   Determination of MICs
b-   Determination of MLS phenotype
c-   Determination of MLS genotype (PCR)


(X)(2)
 


X


X
X
X


X
X
X


(X)(4)
(X)(4)
(X)(4)
5-PCR GENES CODING FOR PILI PROTEINS
 
 
 
X
X
X
6- PCR GENES CODING FOR NON-PILI SURFACE PROTEINS
 
 
 
X
X
X

 

 

 

(1): Epidemiological and microbial surveillance of GBS healthy carriers are organized periodically.  Laboratories are invited to participate in specific protocols in which all details concerning selection criteria, instructions for sampling, for storage and transportation are provided.
(2):  Identification is confirmed for all strains submitted to NRC for any purpose.
(3): CPS typing is performed at time of request for invasive isolates and results are also recorded for further analysis according to epidemiological and microbial surveillance studies.
(4):  AST could be performed according to the validation study protocol.

 

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Inhoudstype: Item
Versie: 2.0
Gemaakt om 30-3-2012 9:29 door Muyldermans, Gaetan
Laatst gewijzigd op 29-1-2013 18:12 door Muyldermans, Gaetan